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- Weaning of three hybridoma cell lines to serum free low protein medium | SpringerLink.
Analytical and Bioanalytical Chemistry [04 Jul , 5 ]. Type: Research Support, Non-U. Abstract Monoclonal antibodies against zearalenone ZEA were raised in mice according to the hybridoma technology and applied in different immunochemical techniques.
Immunochemical techniques. Part I: Hybridoma technology and monoclonal antibodies.
More specifically, three formats based on the competitive direct enzyme immunoassay principle were developed: an enzyme-linked immunosorbent assay ELISA , a flow-through gel-based immunoassay column and a flow-through membrane-based immunoassay. Assay time of both non-instrumental tests was 25 min for 10 samples.
Potentials and limitations of the developed methods were compared. The possible application for multi-mycotoxin analysis of the ELISA method based on a single monoclonal antibody was investigated.
Therefore, principal component analysis and partial least squares regression data modelling were used to separate the immunoassay responses of two cross-reactants ZEA and alpha-ZOL. Read Article at publisher's site.
No loss of antibody production was observed with any of the cell lines. The weaning procedure outlined is both simple and rapid and has been successfully adopted in our laboratory by relatively inexperienced cell culture technicians. Unable to display preview. Download preview PDF.
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Weaning of three hybridoma cell lines to serum free low protein medium. Authors Authors and affiliations K. Radford W. Niloperbowo S. Reid P. Original Research Paper. This is a preview of subscription content, log in to check access. Courier International Ltd, Tiptree, Essex, — Google Scholar. Brown, BL Reducing costs upfront: Two methods for adapting hybridoma cells to an inexpensive, chemically defined serum-free medium.
Seaver ed. Marcel Dekker, Inc. The adaptation of hybridoma cell lines to grow and secrete monoclonal antibody in serum-free defined medium. Immunological Meth. Fleming, JO and Pen, LB Measurement of the concentration of murine IgG monoclonal antibody in hybridoma supernatants and ascites in absolute units by sensitive and reliable enzyme-linked immunosorbent assays Elisa.
Immunological Meth 11— Galfre, G and Milstein, C Preparation of monoclonal antibodies: strategies and procedures. In: Methods in Enzymology, Vol.
Academic Press, New York, 1— Biotechnology and Bioengin. Standard, — Bio Techniques, 8: 76— Jager, V, Lehmann, J and Friedl, P Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors. Cytotechnology 1: — Jayme, DW Alternatives to fetal bovine serum for mammalian cell culture. Focus 3—8. Litwin, J The growth of hybridoma cells in a serum and protein free medium.